LAMP Detection of Four Plant-Pathogenic Oomycetes and Its Application in Lettuce Fields.
Identifieur interne : 000465 ( Main/Exploration ); précédent : 000464; suivant : 000466LAMP Detection of Four Plant-Pathogenic Oomycetes and Its Application in Lettuce Fields.
Auteurs : Wenzhuo Feng [Japon] ; Ayaka Hieno [Japon] ; Mikio Kusunoki [Japon] ; Haruhisa Suga [Japon] ; Koji Kageyama [Japon]Source :
- Plant disease [ 0191-2917 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- méthodes : Agriculture.
- parasitologie : Laitue, Sol.
- Japon, Phytophthora, Pythium, Techniques d'amplification d'acides nucléiques.
- Wicri :
- geographic : Japon.
English descriptors
- KwdEn :
- MESH :
- chemical , parasitology : Soil.
- geographic : Japan.
- methods : Agriculture.
- parasitology : Lettuce.
- Nucleic Acid Amplification Techniques, Phytophthora, Pythium.
Abstract
In Kagawa Prefecture, Japan, the pathogens Phytophthora pseudolactucae, Pythium irregulare, Pythium uncinulatum, and Pythium spinosum have caused huge losses in lettuce production. We used loop-mediated isothermal amplification (LAMP) to analyze soil and plants in lettuce fields for the presence of these four pathogens. To develop an effective on-site detection method, we contrasted the Plant-LAMP and Plant Culture-LAMP procedures for plant samples, and five soil DNA extraction methods for soil samples. Plant-LAMP and a Soil DNA Isolation kit were selected to analyze three fields for the pathogen species present, infected sites, and level of soil contamination. We found that the same wilting symptoms could be caused by Phytophthora or Pythium, or a mixture of species from both genera. Ph. pseudolactucae infects the pith of the lettuce in aboveground parts, whereas Pythium spp. mainly infect roots. Ph. pseudolactucae and Py. uncinulatum caused disease more frequently than the other two pathogens. Furthermore, not all of the pathogens existed in the soil near infected lettuce plants. Therefore, the LAMP method can be used to diagnose pathogenic oomycetes in the field, and will be useful in the development of control strategies in lettuce production.
DOI: 10.1094/PDIS-05-18-0858-RE
PubMed: 30608214
Affiliations:
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(parasitologie)</term><term>Techniques d'amplification d'acides nucléiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="parasitology" xml:lang="en"><term>Soil</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Japan</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Agriculture</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Agriculture</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Laitue</term><term>Sol</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Lettuce</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Nucleic Acid Amplification Techniques</term><term>Phytophthora</term><term>Pythium</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Japon</term><term>Phytophthora</term><term>Pythium</term><term>Techniques d'amplification d'acides nucléiques</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Japon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In Kagawa Prefecture, Japan, the pathogens Phytophthora pseudolactucae, Pythium irregulare, Pythium uncinulatum, and Pythium spinosum have caused huge losses in lettuce production. We used loop-mediated isothermal amplification (LAMP) to analyze soil and plants in lettuce fields for the presence of these four pathogens. To develop an effective on-site detection method, we contrasted the Plant-LAMP and Plant Culture-LAMP procedures for plant samples, and five soil DNA extraction methods for soil samples. Plant-LAMP and a Soil DNA Isolation kit were selected to analyze three fields for the pathogen species present, infected sites, and level of soil contamination. We found that the same wilting symptoms could be caused by Phytophthora or Pythium, or a mixture of species from both genera. Ph. pseudolactucae infects the pith of the lettuce in aboveground parts, whereas Pythium spp. mainly infect roots. Ph. pseudolactucae and Py. uncinulatum caused disease more frequently than the other two pathogens. Furthermore, not all of the pathogens existed in the soil near infected lettuce plants. Therefore, the LAMP method can be used to diagnose pathogenic oomycetes in the field, and will be useful in the development of control strategies in lettuce production.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">30608214</PMID><DateCompleted><Year>2019</Year><Month>07</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>103</Volume><Issue>2</Issue><PubDate><Year>2019</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>LAMP Detection of Four Plant-Pathogenic Oomycetes and Its Application in Lettuce Fields.</ArticleTitle><Pagination><MedlinePgn>298-307</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-05-18-0858-RE</ELocationID><Abstract><AbstractText>In Kagawa Prefecture, Japan, the pathogens Phytophthora pseudolactucae, Pythium irregulare, Pythium uncinulatum, and Pythium spinosum have caused huge losses in lettuce production. We used loop-mediated isothermal amplification (LAMP) to analyze soil and plants in lettuce fields for the presence of these four pathogens. To develop an effective on-site detection method, we contrasted the Plant-LAMP and Plant Culture-LAMP procedures for plant samples, and five soil DNA extraction methods for soil samples. Plant-LAMP and a Soil DNA Isolation kit were selected to analyze three fields for the pathogen species present, infected sites, and level of soil contamination. We found that the same wilting symptoms could be caused by Phytophthora or Pythium, or a mixture of species from both genera. Ph. pseudolactucae infects the pith of the lettuce in aboveground parts, whereas Pythium spp. mainly infect roots. Ph. pseudolactucae and Py. uncinulatum caused disease more frequently than the other two pathogens. Furthermore, not all of the pathogens existed in the soil near infected lettuce plants. Therefore, the LAMP method can be used to diagnose pathogenic oomycetes in the field, and will be useful in the development of control strategies in lettuce production.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Wenzhuo</ForeName><Initials>W</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6114-2051</Identifier><AffiliationInfo><Affiliation>The United Graduate School of Agriculture Science, Gifu University, Gifu 501-1193, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hieno</LastName><ForeName>Ayaka</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>River Basin Research Center, Gifu University, Gifu 501-1193, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kusunoki</LastName><ForeName>Mikio</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Kagawa Prefectural Agricultural Experiment Station Plant Protection Office, Kagawa 761-2306, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suga</LastName><ForeName>Haruhisa</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Life Science Research Center, Gifu University, Gifu 501-1193, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kageyama</LastName><ForeName>Koji</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>River Basin Research Center, Gifu University, Gifu 501-1193, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>01</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000383" MajorTopicYN="N">Agriculture</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007564" MajorTopicYN="N" Type="Geographic">Japan</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018545" MajorTopicYN="Y">Lettuce</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021141" MajorTopicYN="Y">Nucleic Acid Amplification Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="Y">Phytophthora</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011775" MajorTopicYN="Y">Pythium</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>7</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30608214</ArticleId><ArticleId IdType="doi">10.1094/PDIS-05-18-0858-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Préfecture de Gifu</li><li>Région du Chūbu</li></region><settlement><li>Gifu (ville)</li></settlement><orgName><li>Université de Gifu</li></orgName></list><tree><country name="Japon"><region name="Région du Chūbu"><name sortKey="Feng, Wenzhuo" sort="Feng, Wenzhuo" uniqKey="Feng W" first="Wenzhuo" last="Feng">Wenzhuo Feng</name></region><name sortKey="Hieno, Ayaka" sort="Hieno, Ayaka" uniqKey="Hieno A" first="Ayaka" last="Hieno">Ayaka Hieno</name><name sortKey="Kageyama, Koji" sort="Kageyama, Koji" uniqKey="Kageyama K" first="Koji" last="Kageyama">Koji Kageyama</name><name sortKey="Kusunoki, Mikio" sort="Kusunoki, Mikio" uniqKey="Kusunoki M" first="Mikio" last="Kusunoki">Mikio Kusunoki</name><name sortKey="Suga, Haruhisa" sort="Suga, Haruhisa" uniqKey="Suga H" first="Haruhisa" last="Suga">Haruhisa Suga</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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